Project name: Beckam_Project_000
Sample name: Beckman_Pool_1
Run name: r64135_20220101_1111111-1_A01
Movie length: 10 hours
HiFi Reads: 2,231,469
HiFi Yield: 16,007,172,794 bp
HiFi Read Length (mean): 7,129 bp
HiFi Read Quality (median): Q35
HiFi Number of Passes (mean): 13
Report generated: Fri Oct 1 11:01:01 CDT 2022

CCS reads

Demultiplexed HiFi CCS reads

Reads per Sample

The number of Hifi CCS reads per sample is shown. The number of HiFi CCS reads for which a sample barcode could not be determined is shown as “Undetermined”

Mean Read Length per Sample

The Mean length of HiFI CCS reads for each sample is shown.

Data

Starting with SMRT Link v10.0 the primary output of a Sequel II run is a reads.bam file which contains CCS reads of all qualities. Details are available in PacBio’s CCS FAQ. Also provided is a hifi_reads.fastq.gz file containing the high-quality CCS reads (>=Q20). The raw Sequel run folder containing the subreads.bam file is available and can be delivered on request. The raw run folder is required for base modification (methylation) analysis. The hifi CCS reads were demultiplexed with the smrtlink lima software, a fastq.gz file is provided for each sample.